How do I reconstitute a peptide?

Draw bacteriostatic water into a syringe, then slowly inject it down the inside wall of the peptide vial. Do not shake.

How much bacteriostatic water should I use?

Common amounts are 1mL or 2mL. More water creates a lower concentration.

What syringe size should I use?

Insulin syringes (0.3mL/30u, 0.5mL/50u, or 1mL/100u).

How long do reconstituted peptides last?

Refrigerated at 2-8C, typically 2-4 weeks.

Can I use regular water?

No. Bacteriostatic water contains benzyl alcohol to prevent bacterial growth.

How many units should I draw?

Enter your vial size, water volume, and desired dose above.

What does IU mean vs syringe units?

Syringe units are barrel markings (100u = 1mL). IU is a biological measurement.

Peptide Calculator — Free Reconstitution & Dosage Calculator

Reference

Peptide dosage chart

Reference chart for common peptides with typical research dosages.

Peptide	Vial	Typical Dose	Frequency	Category	Cycle Length	Administration
BPC-157	5mg	250-500 mcg	1-2x daily	Healing	4-6 weeks	Subcutaneous near injury site
TB-500	5mg	2,000-5,000 mcg	2x weekly	Healing	4-8 weeks	Subcutaneous, any site
Ipamorelin	5mg	200-300 mcg	2-3x daily	GH Release	8-12 weeks	Subcutaneous, rotate sites
CJC-1295	2mg	100-300 mcg	1-3x daily	GH Release	8-12 weeks	Subcutaneous, abdomen
GHK-Cu	5mg	200-600 mcg	1x daily	Anti-aging	4-6 weeks	Subcutaneous or topical
Tirzepatide	5mg	2,500-5,000 mcg	1x weekly	Weight Loss	Ongoing	Subcutaneous, abdomen/thigh
Semaglutide	3mg	250-1,000 mcg	1x weekly	Weight Loss	Ongoing	Subcutaneous, abdomen/thigh
AOD-9604	5mg	300 mcg	1x daily	Fat Loss	12 weeks	Subcutaneous, abdominal
Epithalon	10mg	5,000-10,000 mcg	1x daily	Anti-aging	10-20 days	Subcutaneous or IM
KPV	5mg	500 mcg	1x daily	Anti-inflammatory	4-8 weeks	Subcutaneous or oral
Selank	5mg	250-500 mcg	1-2x daily	Cognitive	2-4 weeks	Intranasal or subcutaneous
Semax	10mg	200-600 mcg	1-2x daily	Cognitive	2-4 weeks	Intranasal preferred
DSIP	5mg	100-200 mcg	Before bed	Sleep	2-4 weeks	Subcutaneous or IM
PT-141	10mg	1,000-2,000 mcg	As needed	Sexual Health	As needed	Subcutaneous, 45 min prior
GHRP-6	5mg	100-300 mcg	2-3x daily	GH Release	8-12 weeks	Subcutaneous, fasted
Hexarelin	5mg	100-200 mcg	2-3x daily	GH Release	8-12 weeks	Subcutaneous, fasted

This chart provides general research reference ranges. Individual protocols vary based on goals, body weight, and experience level. For personalized dosing, use our dedicated calculators: BPC-157 dosage calculator, semaglutide dosage calculator, or tirzepatide dosage calculator.

Equipment

Choosing the right syringe

Selecting the correct insulin syringe is essential for accurate peptide dosing. The syringe size determines the number of units per milliliter and affects how precisely you can measure small doses.

Syringe Size	Total Units	Units per mL	Smallest Graduation	Best For
0.3 mL	30 units	100 u/mL	0.5 unit	Very small doses (under 300 mcg). Highest precision for micro-dosing protocols.
0.5 mL	50 units	100 u/mL	1 unit	Most peptide dosing. Good balance of capacity and readability.
1.0 mL	100 units	100 u/mL	2 units	Higher-volume doses (TB-500, Epithalon, Tirzepatide). Easier to read for large draws.

Tips for accurate injection

Always use insulin syringes

Standard medical syringes have different unit markings. Insulin syringes are calibrated for the small volumes used in peptide research (100 units = 1 mL).

Remove air bubbles before injecting

After drawing your dose, hold the syringe needle-up and tap the barrel gently. Push the plunger slightly to expel any air at the top. Air bubbles reduce dosing accuracy.

Rotate injection sites

Alternate between the abdomen (1-2 inches from navel), outer thigh, and back of upper arm. Rotating prevents tissue irritation and lipodystrophy from repeated injections at the same spot.

Use a new syringe for each injection

Reusing syringes dulls the needle, increases infection risk, and can introduce bacteria into the peptide vial. Single-use insulin syringes are inexpensive and widely available.

Stability

Peptide storage & handling

Proper storage is critical for maintaining peptide potency. Degraded peptides lose effectiveness and can produce inaccurate research results.

State	Storage Temperature	Shelf Life	Notes
Lyophilized (powder)	-20°C (freezer)	2+ years	Most stable form. Keep sealed and away from moisture.
Lyophilized (powder)	2-8°C (refrigerator)	6-12 months	Acceptable for short-term storage before reconstitution.
Reconstituted (liquid)	2-8°C (refrigerator)	2-4 weeks	Standard recommendation. Use bacteriostatic water only.
Reconstituted (liquid)	Room temperature	48-72 hours max	Avoid. Room temperature accelerates degradation significantly.

Storage best practices

Use bacteriostatic water, not sterile water

Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth. Sterile water has no preservative — once opened, bacteria can multiply within hours. If you plan to draw multiple doses over days or weeks, bacteriostatic water is essential. Learn more in our complete reconstitution guide.

Keep vials upright in the refrigerator

Store reconstituted vials upright to keep the rubber stopper dry. Position them away from the freezer compartment to prevent accidental freezing, which can damage peptide structure through ice crystal formation.

Never freeze reconstituted peptides

Freezing a liquid peptide solution causes ice crystals that can shear the molecular bonds, reducing potency. Only unreconstituted (lyophilized) peptides should be frozen for long-term storage.

Protect from light

Many peptides are photosensitive. Store vials in their original box or wrap in aluminum foil. Avoid placing near windows or under direct lighting.

Swab vial tops before every draw

Clean the rubber stopper with a fresh alcohol swab before each use, even if the vial was used earlier the same day. This prevents introducing contaminants that degrade the peptide.

The Peptide Calculator

Calculate your exact dose

How to reconstitute peptides